TY - JOUR
A2 - Ma, Xuehu
AU - Abdul Hamid, Zariyantey
AU - Lin Lin, Winnie Hii
AU - Abdalla, Basma Jibril
AU - Bee Yuen, Ong
AU - Latif, Elda Surhaida
AU - Mohamed, Jamaludin
AU - Rajab, Nor Fadilah
AU - Paik Wah, Chow
AU - Wak Harto, Muhd Khairul Akmal
AU - Budin, Siti Balkis
PY - 2014
DA - 2014/10/21
TI - The Role of Hibiscus sabdariffa L. (Roselle) in Maintenance of Ex Vivo Murine Bone Marrow-Derived Hematopoietic Stem Cells
SP - 258192
VL - 2014
AB - Hematopoietic stem cells- (HSCs-) based therapy requires ex vivo expansion of HSCs prior to therapeutic use. However, ex vivo culture was reported to promote excessive production of reactive oxygen species (ROS), exposing HSCs to oxidative damage. Efforts to overcome this limitation include the use of antioxidants. In this study, the role of Hibiscus sabdariffa L. (Roselle) in maintenance of cultured murine bone marrow-derived HSCs was investigated. Aqueous extract of Roselle was added at varying concentrations (0–1000 ng/mL) for 24 hours to the freshly isolated murine bone marrow cells (BMCs) cultures. Effects of Roselle on cell viability, reactive oxygen species (ROS) production, glutathione (GSH) level, superoxide dismutase (SOD) activity, and DNA damage were investigated. Roselle enhanced the survival (P<0.05) of BMCs at 500 and 1000 ng/mL, increased survival of Sca-1+ cells (HSCs) at 500 ng/mL, and maintained HSCs phenotype as shown from nonremarkable changes of surface marker antigen (Sca-1) expression in all experimental groups. Roselle increased (P<0.05) the GSH level and SOD activity but the level of reactive oxygen species (ROS) was unaffected. Moreover, Roselle showed significant cellular genoprotective potency against H2O2-induced DNA damage. Conclusively, Roselle shows novel property as potential supplement and genoprotectant against oxidative damage to cultured HSCs.
SN - 2356-6140
UR - https://doi.org/10.1155/2014/258192
DO - 10.1155/2014/258192
JF - The Scientific World Journal
PB - Hindawi Publishing Corporation
KW -
ER -