The Role of Hibiscus sabdariffa L. (Roselle) in Maintenance of Ex Vivo Murine Bone Marrow-Derived Hematopoietic Stem Cells
Effect of Roselle supplementation at various concentrations on H2O2-induced DNA damage of cultured BMCs. Pretreatment of cells with 500 and 1000 ng/mL of Roselle for 24 hours conferred significant protection against H2O2-induced DNA damage as measured through the percentage of DNA in tail (b) and tail moment (c) after 100 μM H2O2 exposure for 10 minutes. Representative slides (a) of alkaline comet assay for various treatment groups are as follows: (A) control; (B) 500 ng/mL Roselle; (C) 1000 ng/mL Roselle; (D) 100 μM H2O2; (E) 500 ng/mL + 100 μM H2O2; (F) 1000 ng/mL + 100 μM H2O2. Scale bar: 50 μm. Results are means ± SEM of 3 replicates. Key: [a], compared to control group; [b], compared to 500 ng/mL Roselle; [c], compared to 1000 ng/mL Roselle; [d], compared to 100 μM H2O2; [e], compared to 500 ng/mL Roselle + 100 μM H2O2.