Table of Contents Author Guidelines Submit a Manuscript
The Scientific World Journal
Volume 2014, Article ID 259765, 13 pages
http://dx.doi.org/10.1155/2014/259765
Research Article

Assessment of Cellular Responses after Short- and Long-Term Exposure to Silver Nanoparticles in Human Neuroblastoma (SH-SY5Y) and Astrocytoma (D384) Cells

1Laboratory of Clinical Toxicology, IRCCS Salvatore Maugeri Foundation, Scientific Institute of Pavia Medical Centre, Via Maugeri 10, 27100 Pavia, Italy
2Toxicology Division, Department of Environmental Health, IRCCS Salvatore Maugeri Foundation, Scientific Institute of Pavia Medical Centre, Via Maugeri 10, 27100 Pavia, Italy
3Toxicology Unit, Department of Clinical Surgical, Diagnostic and Pediatric Sciences, Faculty of Medicine and Surgery, University of Pavia, Via Maugeri 10, 27100 Pavia, Italy
4Department of Molecular Medicine, Institute of Biochemistry, University of Pavia, Via Forlanini 6, 27100 Pavia, Italy

Received 18 November 2013; Accepted 29 December 2013; Published 13 February 2014

Academic Editors: B. Soto-Blanco, A. Takagi, and D. N. Tripathi

Copyright © 2014 Teresa Coccini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure: Membrane integrity evaluation after 4-48 h exposure to increasing concentrations of AgNP (1-100 μg/ml). Representative images of randomly selected microscopic fields of cells (SH-SY5Y, D384, or A549) stained with calcein-AM/PI after 4 (A), 24 (B) and 48 (C) h exposure to increasing concentrations (1-100 μg/ml) of AgNPs. Green fluorescence patterns of both cerebral cell lines were similar to the controls at doses ranging 1 to 25 μg/ml at time points (4, 24 and 48 h). A strong decrease in cell viability was observed as evidenced by the presence of numerous red coloured cells (indicating damage to the cell membrane) at the highest doses (50-100 μg/ml). After 24 and 48 h, the cytotoxic effect of AgNPs was exacerbated. Fluorescence images of A549 cells (A, B, C) showed uniformly diffused green fluorescence, and normal cell morphology for all treatment concentrations (1-100 μg/ml), was observed a slight decrease of cell viability after 48 h exposure only (scale bar 100 μm).

  1. Supplementary Figure