Assessment of Cellular Responses after Short- and Long-Term Exposure to Silver Nanoparticles in Human Neuroblastoma (SH-SY5Y) and Astrocytoma (D384) Cells

Table 2

Semiquantitative cell live analysis after 4–48 h exposure to increasing concentrations of AgNPs (1–100 μg/mL). Semiquantitative analysis of selected microscopic fields of SH-SY5Y, D384, and A549 cells after 4, 24, and 48 h exposure to increasing concentrations of AgNPs (1–100 μg/mL), in terms of cell counts and expressed as percentage of live cells (mean ± S.D).

AgNP (μg/mL)	Live cells (%)
	4 h exposure	24 h exposure	48 h exposure
SH-SY5Y cells
1	100	100	100
10	100	100	100
25	100	35.5±9.46^∗	21±5.69^∗
50	0*	0*	0*
100	0*	0*	0*
D384 cells
1	100	100	100
10	100	100	100
25	100	100	100
50	59.67±12.40^∗	41.67±5.11^∗	6.17±3.20^∗
100	4.00±6.20^∗	0*	0*
A549 cells
1	100	100	84.66±4.08
10	100	100	82.00±2.45
25	100	100	81.66±2.87
50	100	100	79.50±2.88
100	100	100	80.83±2.40

Mean of 6 images for each cell type and each dose of AgNPs. *Statistical analysis by ANOVA with Tukey’s test compared to its control (𝑃<0.05).