Research Article

Optimization of Culture Conditions (Sucrose, pH, and Photoperiod) for In Vitro Regeneration and Early Detection of Somaclonal Variation in Ginger Lime (Citrus assamensis)

Figure 1

Various explants cultured on optimum regeneration media (MS media supplemented with 1.5 mgL−1 NAA and 2.0 mgL−1 BAP), maintained under 16 hours of light and 8 hours of darkness, with light intensity of 1000 lux. (a) Formation of yellow callus from root explant, (b) formation of green callus from root explant, (c) formation of green callus and production of shoot primordia from stem explant, (d) formation of roots from leaf explant after 18 days of culture, (e) formation of green callus from leaf explant, and (f) formation of multiple shoots from stem explant.
262710.fig.001a
(a)
262710.fig.001b
(b)
262710.fig.001c
(c)
262710.fig.001d
(d)
262710.fig.001e
(e)
262710.fig.001f
(f)