A Novel In Vitro Protocol for Inducing Direct Somatic Embryogenesis in Phalaenopsis aphrodite without Taking Explants

Table 4

Number of embryos formed on the leaf surface of 4-month-old seedlings, depending on culture systems and concentration of cytokinins.

PGR treatments (mg L^−1)	Solid medium	Two-layer medium	Two-step culture
PGR-free	0^a*	0^c	5.0^b
BA
1	0^a	2.6^bc	10.2^ab
2.5	0^a	3.6^b	12.8^ab
5	0^a	3.0^bc	8.0^b
TDZ
0.1	0^a	5.0^ab	15.2^ab
0.5	0^a	7.0^a	19.2^a
1.0	0^a	4.4^ab	12.6^ab

∗Data in the same column followed by the same letters are not significantly different by Duncan’ multiple range test at P < 0.05.