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The Scientific World Journal
Volume 2014, Article ID 269793, 7 pages
Research Article

In Vitro Phytochemical, Antibacterial, and Antifungal Activities of Leaf, Stem, and Root Extracts of Adiantum capillus veneris

1Department of Microbiology, Faculty of Life Sciences, Abasyn University, Peshawar 25000, Pakistan
2Medicinal Botanic Centre, PCSIR Labs Complex, Peshawar 25120, Pakistan
3Department of Microbiology, Shaheed Benazir Bhutto Women University, Peshawar 25000, Pakistan
4Department of Chemistry, Kohat University of Science and Technology, Kohat 26000, Pakistan

Received 31 August 2013; Accepted 24 November 2013; Published 28 January 2014

Academic Editors: F. Bussotti, M. Chen, R. Creamer, and N. Suganuma

Copyright © 2014 Muhammad Saqib Ishaq et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Adiantum capillus veneris is a medicinally essential plant used for the treatment of diverse infectious diseases. The study of phytochemical and antimicrobial activities of the plant extracts against multidrug-resistant (MDR) bacteria and medically important fungi is of immense significance. Extracts from the leaves, stems, and roots of Adiantum capillus veneris were extracted with water, methanol, ethanol, ethyl acetate, and hexane and screened for their antimicrobial activity against ten MDR bacterial strains and five fungal strains isolated from clinical and water samples. Ash, moisture, and extractive values were determined according to standard protocols. FTIR (Fourier transform infrared Spectroscopy) studies were performed on different phytochemicals isolated from the extracts of Adiantum capillus Veneris. Phytochemical analysis showed the presence of flavonoids, alkaloids, tannins, saponins, cardiac glycosides, terpenoids, steroids, and reducing sugars. Water, methanol, and ethanol extracts of leaves, stems, and roots showed significant antibacterial and antifungal activities against most of the MDR bacterial and fungal strains. This study concluded that extracts of Adiantum capillus veneris have valuable phytochemicals and significant activities against most of the MDR bacterial strains and medically important fungal strains.