Evaluation of Different Culture Media for Improvement in Bioinsecticides Production by Indigenous Bacillus thuringiensis and Their Application against Larvae of Aedes aegypti

Table 2

Comparative analysis of Bt  𝑠𝑣2 production in different nitrogen sources.

Nitrogen source (g/L)	Biomass yield (g/L)	Toxin content (protein mg/L)	Spores (CFU/mL)	Toxin/biomass (mg/g)
BN + ammonium sulphate (5)	5.44 (0.05)^c	16.0 (0.20)^c	7.30×10^7	2.94
BN + sodium nitrate (5)	4.90 (0.10)^d	14.2 (0.30)^e	9.10×10^6	2.89
BN + egg albumin (5)	4.96 (0.50)^d	15.5 (0.20)^c	1.50×10^7	3.12
BN + beef extract (5)	4.70 (0.03)^d	11.3 (0.50)^d	1.40×10^6	2.40
BN + casein (5)	6.67 (0.05)^b	18.4 (0.60)^b	1.69×10^8	2.75
BN + malt extract (5)	5.81 (0.06)^c	15.4 (0.80)^c	2.40×10^8	2.65
BN + soybean (5)	6.54 (0.04)^b	19.7 (0.20)^b	1.12×10^9	3.01
BN + pigeon pea (5)	7.45 (0.09)^a	28.2 (0.70)^a	2.24×10^9	3.78
BN + yeast extract (5)	6.26 (0.08)^b	16.7 (0.40)^c	9.10×10^8	2.66
LB medium (25)	5.40 (0.02)^c	18.8 (0.20)^b	1.32×10^9	3.48

BN: Basal medium for nitrogen source study (NaCl 2.5 gm/L + Na₂HPO₄ 1 gm/L + MgSO₄ 0.2 gm/L + MnCl₂ 0.05 gm/L + glucose 10 gm/L);
data are presented as mean (SD). Means within a given column followed by the same letter are not significantly different, Tukey’s MRT (𝛼=0.05), 𝑃<0.0001.