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The Scientific World Journal
Volume 2014, Article ID 279451, 12 pages
http://dx.doi.org/10.1155/2014/279451
Research Article

Antioxidant Capacity and Radical Scavenging Effect of Polyphenol Rich Mallotus philippenensis Fruit Extract on Human Erythrocytes: An In Vitro Study

1Department of Pharmacology, Faculty of Modern Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
2Department of Microbiology, Faculty of Modern Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
3Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi 21005, India

Received 19 July 2014; Revised 27 October 2014; Accepted 4 November 2014; Published 26 November 2014

Academic Editor: Raffaella Sorrentino

Copyright © 2014 Mayank Gangwar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mallotus philippinensis is an important source of molecules with strong antioxidant activity widely used medicinal plant. Previous studies have highlighted their anticestodal, antibacterial, wound healing activities, and so forth. So, present investigation was designed to evaluate the total antioxidant activity and radical scavenging effect of 50% ethanol fruit glandular hair extract (MPE) and its role on Human Erythrocytes. MPE was tested for phytochemical test followed by its HPLC analysis. Standard antioxidant assays like DPPH, ABTS, hydroxyl, superoxide radical, nitric oxide, and lipid peroxidation assay were determined along with total phenolic and flavonoids content. Results showed that MPE contains the presence of various phytochemicals, with high total phenolic and flavonoid content. HPLC analysis showed the presence of rottlerin, a polyphenolic compound in a very rich quantity. MPE exhibits significant strong scavenging activity on DPPH and ABTS assay. Reducing power showed dose dependent increase in concentration absorption compared to standard, Quercetin. Superoxide, hydroxyl radical, lipid peroxidation, nitric oxide assay showed a comparable scavenging activity compared to its standard. Our finding further provides evidence that Mallotus fruit extract is a potential natural source of antioxidants which have a protective role on human Erythrocytes exhibiting minimum hemolytic activity and this justified its uses in folklore medicines.