Optimization of Aeration and Agitation Rate for Lipid and Gamma Linolenic Acid Production by Cunninghamella bainieri 2A1 in Submerged Fermentation Using Response Surface Methodology

Table 5

Analysis of variance for the quadratic polynomial model of lipid content produced by Cunninghamella bainieri 2A1.

Source	Polynomial coefficients	Sum of squares	DF	Mean square	value	Prob >
Model		300.21	5	60.4	13.84	0.0016^*
Intercept	37.75		1
	1.41	15.83	1	15.83	3.65	0.0978
	3.80	115.74	1	115.74	26.68	0.0013^*
	−1.161	9.30	1	9.30	2.14	0.1865
	−3.86	103.86	1	103.86	23.94	0.0018^*
	−3.93	61.78	1	61.78	14.24	0.0070^*
Residual		30.37	7	4.34
Lack of fit		22.46	3	7.49	3.79	0.1156
Pure error		7.91	4	1.98

Statistically significant at 99% probability level.
: aeration rate (vvm); : agitation rate (rpm); and : the quadratic terms; : the interaction term.
= 0.9081.