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The Scientific World Journal
Volume 2014, Article ID 306217, 10 pages
Research Article

Phenolic Composition and Antioxidant Activity of Malus domestica Leaves

1Department of Pharmacognosy, Faculty of Pharmacy, Lithuanian University of Health Sciences, Eivenių Street 4, 50161 Kaunas, Lithuania
2Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kauno Street 30, Babtai, 54333 Kaunas, Lithuania

Received 2 June 2014; Revised 11 August 2014; Accepted 25 August 2014; Published 11 September 2014

Academic Editor: Wanchai De-Eknamkul

Copyright © 2014 Mindaugas Liaudanskas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to determine the composition and content of phenolic compounds in the ethanol extracts of apple leaves and to evaluate the antioxidant activity of these extracts. The total phenolic content was determined spectrophotometrically, as well as the total flavonoid content in the ethanol extracts of apple leaves and the antioxidant activity of these extracts, by the ABTS, DPPH, and FRAP assays. The highest amount of phenolic compounds and flavonoids as well as the highest antioxidant activity was determined in the ethanol extracts obtained from the apple leaves of the cv. Aldas. The analysis by the HPLC method revealed that phloridzin was a predominant component in the ethanol extracts of the apple leaves of all cultivars investigated. The following quercetin glycosides were identified and quantified in the ethanol extracts of apple leaves: hyperoside, isoquercitrin, avicularin, rutin, and quercitrin. Quercitrin was the major compound among quercetin glycosides.