Antioxidant Properties of Berberis aetnensis C. Presl (Berberidaceae) Roots Extract and Protective Effects on Astroglial Cell Cultures

Table 1

Effect of B. aetnensis C. Presl chloroformic extract on GSH levels in primary rat astroglial cell cultures at 14 DIV exposed to 500 μM glutamate for 24 hrs.

Treatment	nmoles/mg protein
Control	15 ± 0.9
5 g/mL B. aetnensis C. Presl	15 ± 0.2
10 g/mL B. aetnensis C. Presl	14.8 ± 0.6
20 g/mL B. aetnensis C. Presl	14.7 ± 0.9
Glutamate 500 M	9.5 ± 0.4^a
Glutamate 500 M + 5 g/mL B. aetnensis; C. Presl	10.6 ± 0.3^a
Glutamate 500 M + 10 g/mL B. aetnensis C. Presl	11.20 ± 0.5^ab
Glutamate 500 M + 20 g/mL B. aetnensis C. Presl	14 ± 0.3^b

GSH levels are expressed as nmol of GSH/mg protein. Each value represents the mean ± SD of 5 separate experiments. versus control and ^b versus glutamate.