The Scientific World Journal / 2014 / Article / Tab 2

Research Article

Antioxidant Properties of Berberis aetnensis C. Presl (Berberidaceae) Roots Extract and Protective Effects on Astroglial Cell Cultures

Table 2

Effect of B. aetnensis C. Presl chloroformic extract on ROS levels in primary rat astroglial cell cultures at 14 DIV exposed to 500 μM glutamate for 24 hrs.

TreatmentI.F./mg protein

Control3.8 ± 0.52
5 g/mL B. aetnensis C. Presl3.75 ± 0.6
10 g/mL B. aetnensis C. Presl3.81 ± 0.45
20 g/mL B. aetnensis C. Presl3.78 ± 0.55
Glutamate 500 M6.8 ± 0.9a
Glutamate 500 M + 5 g/mL B. aetnensis C. Presl5.2 ± 0.5ab
Glutamate 500 M + 10 g/mL B. aetnensis C. Presl4.6 ± 0.7ab
Glutamate 500 M + 20 g/mL B. aetnensis C. Presl4 ± 0.5b

ROS levels are expressed as nmol of dichlorofluorescein produced 30 min/mg protein. Each value represents the mean ± SD of 5 separate experiments. versus control and b versus glutamate.