High Level Aminoglycoside Resistance and Distribution of Aminoglycoside Resistant Genes among Clinical Isolates of Enterococcus Species in Chennai, India

Table 1

Primers and their sequences for aminoglycoside resistance encoding genes used in multiplex PCR.

Genes	Primer sequences (5′-3′)		Size of PCR product (bp)
aac(6′ )-Ie-aph(2′′)-Ia	F: CAGGAATTTATCGAAAATGGTAGAAAAG	R: CACAATCGACTAAAGAGTACCAATC	369
aph(2′′ )-Ib	F: CTTGGACGCTGAGATATATGAGCAC	R: GTTTGTAGCAATTCAGAAACACCCTT	867
aph(2′′ )-Ic	F: CCACAATGATAATGACTCAGTTCCC	R: CCACAGCTTCCGATAGCAAGAG	444
aph(2′′ )-Id	F: GTGGTTTTTACAGGAATGCCATC	R: CCCTCTTCATACCAATCCATATAACC	641
aph(3′ )-IIIa	F: GGCTAAAATGAGAATATCACCGG	R: CTTTAAAAAATCATACAGCTCGCG	523