Validation of Novel Reference Genes for Reverse Transcription Quantitative Real-Time PCR in Drought-Stressed Sugarcane
Figure 3
Relative expression of glutamine-dependent asparagine synthetase (AS), pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFPα1), plasma membrane intrinsic protein 1-1 (PIP1-1), and 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase) by the REST software v. 2.0.13 (after the ΔΔCq method) in cDNAs of sugarcane roots under abiotic stress (24 h of continuous dehydration or salt stress; 100 mM NaCl), normalized by the reference genes H1, αTUB, and GAPDH. (a) Tolerant bulk (CTC6, CTC15, SP83-2847, and SP83-5073 accessions) compared to its negative control. (b) Susceptible bulk (CTC9, CTC13, SP90-1638, and SP90-3414) compared to its negative control. (c) Salt-tolerant accession (RB931011) after 30 min of salt stress compared to its negative control. (d) RB931011 after 90 min of salt stress compared to its negative control. Relative expression with the median value (horizontal dotted line at the colored box) and range comprising 100% of the observations (horizontal bars), being 50% of them in the confidence interval at 95% (colored box).
