Arsenic Modulates Posttranslational S-Nitrosylation and Translational Proteome in Keratinocytes
Figure 1
The arsenic decreases protein S-nitrosylation in primary keratinocytes. (a) The scheme of biotin switch showed that the NO on the cysteine residue was replaced by biotin. (b) After biotin switch, 40 μg of biotinylated lysates treated with 10 μM of arsenic for 1 h was separated by SDS-PAGE. The blotted membrane was prestained by Ponceau S and western blotted with actin (1 : 3000). Both two were applied as loading control. Streptavidin-HRP (1 : 3000) was applied to detect biotinylated proteins. The decreased S-nitrosylated proteins after arsenic treatment were indicated as triangle.
