Research Article
Production and Purification of a Novel Xanthan Lyase from a Xanthan-Degrading Microbacterium sp. Strain XT11
Table 1
Purification of xanthan lyase from the xanthan-grown culture of Microbacterium sp. XT11.
| | Purification steps | Lyase activity (U) | Total protein content (mg) | Specific activity (U/mg) | Yield
% | Purification fold |
| | Cell-free culture broth | 253.3 | 115 | 2.2 | 100 | 1 | | (NH4)2SO4 precipitation | 191.4 | 71.3 | 2.7 | 75.6 | 1.2 | | Phenyl Sepharose | 150.3 | 28.4 | 5.3 | 59.3 | 2.4 | | Q Sepharose FF | 126.7 | 4.5 | 28.2 | 50.4 | 12.8 |
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