Aromatic Amino Acid Mutagenesis at the Substrate Binding Pocket of Yarrowia lipolytica Lipase Lip2 Affects Its Activity and Thermostability
Figure 4
The Lineweaver-Burk plot of the purified lipases. Lipase activity was detected at 40°C in 50 mmol pBS (pH 8.0) using pNPC16 with different concentrations (0.2–1.0 mmol) as substrate. (a), (b), and (c) showed the Lineweaver-Burk plot of WT, V94W, and I100F.
