Aromatic Amino Acid Mutagenesis at the Substrate Binding Pocket of Yarrowia lipolytica Lipase Lip2 Affects Its Activity and Thermostability

Table 1

Substrate specificity of WT, V94W, and I100F under standard conditions.

Substrates	WT activity (U mg^−1)	V94W activity (U mg^−1)	I100F activity (U mg^−1)
-Nitrophenyl acetate
-Nitrophenyl butyrin
-Nitrophenyl caprylate
-Nitrophenyl caprate
-Nitrophenyl laurate
-Nitrophenyl myristate
-Nitrophenyl palmitate

*The activities were detected in 50 mmol PBS (pH 8.0) at 40°C. WT means the wild-type enzyme (the amino acid residual in position 94 is V and in 100 is I). The values represent the means of three independent experiments (mean ± SD).