Aromatic Amino Acid Mutagenesis at the Substrate Binding Pocket of Yarrowia lipolytica Lipase Lip2 Affects Its Activity and Thermostability
Substrate specificity of WT, V94W, and I100F under standard conditions.
WT activity (U mg−1)
V94W activity (U mg−1)
I100F activity (U mg−1)
*The activities were detected in 50 mmol PBS (pH 8.0) at 40°C. WT means the wild-type enzyme (the amino acid residual in position 94 is V and in 100 is I). The values represent the means of three independent experiments (mean ± SD).