The Scientific World Journal / 2014 / Article / Tab 2

Research Article

Aromatic Amino Acid Mutagenesis at the Substrate Binding Pocket of Yarrowia lipolytica Lipase Lip2 Affects Its Activity and Thermostability

Table 2

Specific activity and kinetics parameters of wild-type enzyme and mutant enzymes.

WT V94W I100F

Specific activity (U mg−1)
(mmol)
(μmol min−1)
(s−1)
/ (mmol−1 s−1)

*The activities were tested in 50 mmol pH 8.0 PBS at 40°C. The values represent the means of three independent experiments (mean ± SD).