Evaluation of the Cytotoxic Effects of CAM Therapies: An In Vitro Study in Normal Kidney Cell Lines

Figure 4

Evaluation of apoptosis in NRK-52E cells by AO/EB staining after 48 hours of treatment with IC₅₀ values of HWE, CWE, MWE, AWE, EAA, AAA, PAE, AAE, MAE, 30 C, and MT of Ruta and 5-FU. Cells were observed under fluorescence microscope at 200x. (A) Control (untreated); (C) 5-FU; (E) HWE; (G) CWE; (I) MWE; (K) AWE; (M) EAA; (O) AAA; (Q) PAE; (S) AAE; (U) MAE; (W) 30 C; (Y) MT were observed under acridine orange filter and (B) control (untreated); (D) 5-FU; (F) HWE; (H) CWE; (J) MWE; (L) AWE; (N) EAA; (P) AAA; (R) PAE; (T) AAE; (V) MAE; (X) 30 C (Z) MT were observed under ethidium bromide filter.