Research Article
Validation of Simultaneous Quantitative Method of HIV Protease Inhibitors Atazanavir, Darunavir and Ritonavir in Human Plasma by UPLC-MS/MS
Table 1
Optimized mass spectrometer parameters, MRM transitions, and chromatographic performance.
| | Parameters | ATV | ATV-d6 | DRV | DRV-d9 | RTV | RTV-d6 |
| | Mass spectrometry parameters |
| | Source dependent | | Capillary voltage (kV) | | | 4.0 | | | | Extractor voltage (V) | | | 3.0 | | | | RF lens (V) | | | 0.0 | | | | Source temperature (°C) | | | 110 | | | | Desolvation temperature (°C) | | | 400 | | | | Desolvation gas flow (L/h) | | | | | | | Cone gas flow (L/h) | | | | | | | Analyzer parameters | | | | | | | | LM 1/HM 1 resolution | | | 15.0/15.0 | | | | Ion energy 1/ion energy 2 | | | 0.2/1.0 | | | | Entrance/exit | | | −1.0/0.1 | | | | LM 2/HM 2 resolution | | | 14.0/14.0 | | |
| | Compound dependent | | Cone voltage (V) | 30 | 29 | 25 | 27 | 30 | 26 | | Collision energy (eV) | 44 | 42 | 17 | 17 | 20 | 21 | | Dwell time (ms) | 200 | 200 | 200 | 200 | 200 | 200 | | MRM transition (m/z) | 705.3/167.9 | 711.2/168.0 | 548.1/392.0 | 557.1/401.0 | 721.3/296.3 | 727.4/302.3 |
| | Chromatography characteristics |
| | Retention time (min) | 0.69 | 0.69 | 1.02 | 1.01 | 1.54 | 1.54 | | Capacity factors | 1.15 | 1.16 | 2.18 | 2.15 | 3.81 | 3.82 | | Theoretical plates | 396 | 398 | 851 | 836 | 1683 | 1682 |
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ATV: atazanavir; ATV-d6: atazanavir-d6; DRV: darunavir; DRV-d9: darunavir-d9; RTV: ritonavir; RTV-d6: ritonavir-d6.
RF: radio frequency; LM: low mass; HM: high mass.
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