The Scientific World Journal / 2014 / Article / Tab 2

Research Article

Effects of Dimethylaminoethanol and Compound Amino Acid on D-Galactose Induced Skin Aging Model of Rat

Table 2

Primer pairs for target and housekeeping genes for quantitative RT-PCR assay.

Target gene 
accession number
SequencesProbe length
ForwardReverse

Col 1 I 
NM_053304.1
ATGTCTGGTTTGGA 
GAGAGCA 
GAGGAGCAGGGAC 
TTCTTGAG 
203 bp 
Col 1 III 
NM_032085.1
GCCTCCCAGAACAT 
TACATACC 
TTTGCTATTTCCTTC 
AGCCTTG 
132 bp 
MMP-1
NM_001134530.1
CTCCCTTGGACTCA 
CTCATTCTA 
AGAACATCACCTCT 
CCCCTAAAC 
227 bp 
TIMP-1 
NM_053819.1
TGGCATCCTCTTGTT 
GCTATC 
CGAATCCTTTGAGC 
ATCTTAGTC 
191 bp 
-Action 
NM_031144.3
GGAGATTACTGCCC 
TGGCTCCTA
GACTCATCGTACTC 
CTGCTTGCTG
150 bp

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