Effects of Dimethylaminoethanol and Compound Amino Acid on D-Galactose Induced Skin Aging Model of Rat

Table 2

Primer pairs for target and housekeeping genes for quantitative RT-PCR assay.

Target gene; accession number	Sequences		Probe length
	Forward	Reverse
Col 𝛼1 I; NM_053304.1	ATGTCTGGTTTGGA; GAGAGCA	GAGGAGCAGGGAC; TTCTTGAG	203 bp
Col 𝛼1 III; NM_032085.1	GCCTCCCAGAACAT; TACATACC	TTTGCTATTTCCTTC; AGCCTTG	132 bp
MMP-1𝛼; NM_001134530.1	CTCCCTTGGACTCA; CTCATTCTA	AGAACATCACCTCT; CCCCTAAAC	227 bp
TIMP-1; NM_053819.1	TGGCATCCTCTTGTT; GCTATC	CGAATCCTTTGAGC; ATCTTAGTC	191 bp
𝛽-Action; NM_031144.3	GGAGATTACTGCCC; TGGCTCCTA	GACTCATCGTACTC; CTGCTTGCTG	150 bp