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The Scientific World Journal
Volume 2014, Article ID 514835, 6 pages
http://dx.doi.org/10.1155/2014/514835
Research Article

Effect of Codon Optimisation on the Production of Recombinant Fish Growth Hormone in Pichia pastoris

1Institute of Research Management & Monitoring, Deputy Vice Chancellor (Research & Innovation) Building, University of Malaya, 50603 Kuala Lumpur, Malaysia
2Genetics and Molecular Biology Unit, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

Received 24 April 2014; Accepted 9 July 2014; Published 22 July 2014

Academic Editor: Héla Kallel

Copyright © 2014 Hussin A. Rothan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was established to test the hypothesis of whether the codon optimization of fish growth hormone gene (FGH) based on P. pastoris preferred codon will improve the quantity of secreted rFGH in culture supernatant that can directly be used as fish feed supplements. The optimized FGH coding sequence (oFGH) and native sequence (nFGH) of giant grouper fish (Epinephelus lanceolatus) were cloned into P. pastoris expression vector (pPICZαA) downstream of alcohol oxidase gene (AOX1) for efficient induction of extracellular rFGH by adding 1% of absolute methanol. The results showed that recombinant P. pastoris was able to produce  mg of oFGH compared to of nFGH in one litre of culture supernatant. The total body weight of tiger grouper fingerlings fed with oFGH increased significantly at third ( ) and fourth weeks ( ) of four-week experiment period compared to those fed with nFGH. Both oFGH and nFGH significantly enhanced the final biomass and fish survival percentage. In conclusion, codon optimization of FGH fragment was useful to increase rFGH quantity in the culture supernatant of P. pastoris that can be directly used as fish feed supplements. Further studies are still required for large scale production of rFGH and practical application in aquaculture production.