Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization in Iris. lactea var. chinensis Roots under Cadmium, Lead, and Salt Stress Conditions

Table 1

Description of Iris. lactea var. chinensis reference genes for RT-qPCR.

Gene^a	NCBI accession number	Arabidopsis ortholog locus^b	Arabidopsis locus description
UBC	EX953716	AT4g27960	Ubiquitin-protein ligase UBC9
TUBLIN	EX954248	AT5G19780	Tubulin alpha-5
EIF-5A	EX954588	AT1G69410	Eukaryotic translation initiation factor
EF1α	EX950257	AT5G60390	Translation elongation factor EF1A
EF1b	AB907790	AT2G18110	Translation elongation factor EF1B
ACTIN	EX952640	AT3G12110	ACT11
HIS	FD387291	AT4G40040	Histone H3

aAll genes were named on the basis of similarity to Arabidopsis proteins determined via BLASTX.
^bClosest Arabidopsis homolog identified using TAIR BLAST (http://www.arabidopsis.org/Blast/index.jsp).