Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A

Table 1

Comparison of FACS with SYBR Green I and Giemsa stained microscopic analyses for the determination of malaria parasitemia.

Expected parasitemia (%)	Observed value						FACS/microscopy (%)
	Light microscopy			FACS
	Mean	SD	CV (%)	Mean	SD	CV (%)
22	22.05	1.59	7.20%	23.68	0.53	2.20%	107%
10	8.23	0.69	8.40%	12.45	0.37	3.00%	151%
5	4.51	0.92	20.50%	5.63	0.25	4.40%	125%
1	0.89	0.17	18.70%	1.18	0.13	10.70%	132%
0.5	0.52	0.05	9.00%	0.68	0.1	14.60%	132%
0.1	0.17	0.06	33.70%	0.15	0.02	16.30%	91%
0.01	0.01	0.0096	66.00%	0.02	0.0062	37.20%	116%