The p85 Regulatory Subunit of PI3K Mediates cAMP-PKA and Insulin Biological Effects on MCF-7 Cell Growth and Motility
Figure 5
Role of phosphorylation of S83 in the control of migratory behaviour. Wound healing assay on MCF-7 cells overexpressing p85WT or mutants untreated or treated with insulin 10 nM for 12 h. The white lines indicate the limits of the wound; the black lines indicate the limits after 12 h in the presence of 0.5% FCS or in the presence of 10 nM insulin. The red lines in the lower part of the upper panel represent the predicted limits of the wound without insulin. Histogram represents quantification of the insulin treatment effect on cell motility (% of wound healing) calculated as ratio between the space delimited by the white lines and those delimited by the black ones; ( comparing versus empty vector). Images are representative of three separate experiments performed in triplicate (). These results were confirmed by a transwell migration assay (data not shown).
