Agrobacterium-Mediated Transformation of the Recalcitrant Vanda Kasem's Delight Orchid with Higher Efficiency
Figure 2
Schematic diagram of the plasmid used for optimization and transformation studies. (a) The binary vector pCAMBIA 1304 (CSIRO, Australia) harboring the reporter gusA and mgfp5 genes driven by the CaMV 35S promoter. (b) Recombinant plasmids pW2KY and pW1B1 containing wwin2 and wwin1 genes, respectively. RB: right border, LB: left border, nos promoter: nopaline synthase promoter, nosT: nopaline synthase terminator, nptII: neomycin phosphotransferase resistance gene, nos poly(A): nopaline synthase polyadenylation signal, wwin1: coding region of the PR4, wwin2: coding region of the PR4, 35S promoter: cauliflower mosaic virus (CaMV) 35S promoter, and rbcS poly(A): ribulose-1,5-bisphosphate carboxylase small subunit gene polyadenylation signal. Relevant restriction sites are also indicated.
