Characterization of the algC Gene Expression Pattern in the Multidrug Resistant Acinetobacter baumannii AIIMS 7 and Correlation with Biofilm Development on Abiotic Surface

Table 1

Oligos/probes used for PCR, RT-PCR, and real-time PCR analysis.

Gene	Primer	5′-3′ Nucleotide sequence
algC	118_F	TTAGAACCGGGTGAGCGTTTAGCA
	1897_R	AGATGCTGATCTTGTGGCATTGCG
	ALGC1_F	GCTGTGAAGTCATTGCTTTACGT
	ALGC1_R	TGAAGGGTCTGGTGCATGATC
	ALGC1_MFAM	6FAM-CAAATGGTGAGTTCCC-TAMRA
algC cDNA int_region  1	CD363F	CCGTGCTTACGATATTAGAGGCAA
	CD1701R	AATTTGCGGATAACGCTCTTGC
algC cDNA int_region  2	1260nest1	CTTCCTCCGCGCGTATTTATC
	CD1701R	AATTTGCGGATAACGCTCTTGC
16SrRNA	16B-F	TGGCTCAGATTGAACGCTGGCGGC
	16B-R	TACCTTGTTACGACTTCACCCCA
	16SRR_F	TGTCGTGAGATGTTGGGTTAAGTC
	16SRR_R	CCGAAATGCTGGCAAGTAAGGA
	16SRR_MFAM	6FAM-ACGAGCGCAACCCTTT-TAMRA

Primers for real-time PCR assays.