Activity of a Carboxyl-Terminal Truncated Form of Catechol 2,3-Dioxygenase from Planococcus sp. S5

Table 3

Kinetic parameters for catechol oxygenation to 2-hydroxymuconic semialdehyde by wild-type (wt) and mutant C23O dioxygenases.

Enzyme/substrate	C23Owt		C23OB61
	𝐾𝑚 (µM)	𝑉max (mU/mg)	𝐾𝑚 (µM)	𝑉max (mU/mg)
Catechol	97.74±10.91	115.38±14.40	66.17±9.44	104.50±8.43

All data shown were expressed as mean ± standard deviation (𝑛=4).