Research Article

Automated Long-Term Monitoring of Parallel Microfluidic Operations Applying a Machine Vision-Assisted Positioning Method

Figure 4

Defined geometry of the alignment mark. (a) Position of alignment mark next to a culture chamber (left), extracted edges of the alignment mark (middle), and two template images used in the convolution for alignment mark detection (right) in the template-matching procedures. Scale bar: 100 μm. (b) Generated maps of different matching schemes between the template image and the alignment mark image. We show here a captured microscopic image (lower) and an ideal-case image (upper) of the alignment mark. Scale bar: 150 μm.