In Vivo Hypoglycaemic Effect and Inhibitory Mechanism of the Branch Bark Extract of the Mulberry on STZ-Induced Diabetic Mice

Table 2

mRNA primer design for the key metabolic enzymes in liver.

Target gene	(5′ to 3′) Primer sequences	Product; length
G6Pas_F	CACCGACTACTACAGCAACAGC	209
G6Pas_R	AGAATCCCAACCACAAGATGAC
GCK_F	CTTCACCTTCTCCTTCCCTGTAA	145
GCK_R	AAAGTCCCCTCTCCTCTTGATAG
PEPCK_F	AGTCATCATCACCCAAGAGC	154
PEPCK_R	TGGGATGACATACATGGTGC
-actin-F	CAGCCTTCCTTCTTGGGTAT	91
-actin-R	GGTCTTTACGGATGTCAACG