In Vivo Hypoglycaemic Effect and Inhibitory Mechanism of the Branch Bark Extract of the Mulberry on STZ-Induced Diabetic Mice

Table 4

mRNA primer design for INS-1, INS-2, and PDX-1 in pancreas.

Target gene	Primer sequences (5′ to 3′)	The length of the product
Insulin-1_F	GTGATAAAACCCTGACAAGAGCA	141
Insulin-1_R	TGTGGGGATAATAGGAGCAGTT
Insulin-2_F	CAAGTGGCACAACTGGAGCT	110
Insulin-2_R	CTGGTGCAGCACTGATCTAC
Pdx-1_F	AGTGGGCAGGAGGTGCTTAC	186
Pdx-1_R	GGAACCAGATTTTGATGTGTCTCT
-Actin-F	CAGCCTTCCTTCTTGGGTAT	91
-Actin-R	GGTCTTTACGGATGTCAACG