Use of Heavy Water (D₂O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Figure 1

Thermostability of rp26 ELISA at 4°C. 𝑃𝑃 value of strong positive serum (■), medium positive serum (●), and weak positive serum (▲) was compared with fresh ELISA. Duration of thermostability is indicated by arrow (↓) against each serum. Beyond this point, significant deviation in 𝑃𝑃 value was observed in comparison to fresh plate (𝑃=0.03). No significant difference exists in 80% D₂O versus H₂O coating buffer, 60% D₂O versus H₂O coating buffer, and 60% D₂O versus 80% D₂O.

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