Use of Heavy Water (D2O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection
Thermostability of rp26 ELISA at 4°C. value of strong positive serum (■), medium positive serum (●), and weak positive serum (▲) was compared with fresh ELISA. Duration of thermostability is indicated by arrow () against each serum. Beyond this point, significant deviation in value was observed in comparison to fresh plate (). No significant difference exists in 80% D2O versus H2O coating buffer, 60% D2O versus H2O coating buffer, and 60% D2O versus 80% D2O.
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