The Scientific World Journal / 2014 / Article / Fig 1

Research Article

Use of Heavy Water (D2O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Figure 1

Thermostability of rp26 ELISA at 4°C. value of strong positive serum (■), medium positive serum (●), and weak positive serum (▲) was compared with fresh ELISA. Duration of thermostability is indicated by arrow ( ) against each serum. Beyond this point, significant deviation in value was observed in comparison to fresh plate ( ). No significant difference exists in 80% D2O versus H2O coating buffer, 60% D2O versus H2O coating buffer, and 60% D2O versus 80% D2O.

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