Differential Diagnosis of Entamoeba spp. in Clinical Stool Samples Using SYBR Green Real-Time Polymerase Chain Reaction

Table 1

Results of multiplex PCR obtained with different DNA concentration.

DNA (pg)		𝐶𝑡 value
E. histolytica	E. dispar
183.5	198.0	23.1
91.5	99.0	23.9
45.9	49.5	25.4
22.9	24.7	26.5
11.5	12.4	27.3
5.7	6.2	28.1
2.9	3.1	29.3
1.4	1.6	30.0
0.7	0.8	31.1
0.36	0.39	31.5