Research Article
Sequence Variants of ADIPOQ and Association with Type 2 Diabetes Mellitus in Taiwan Chinese Han Population
Table 2
Primers utilized in allele-specific PCR analysis.
| | Gene/SNP | Primers, 5′-3′ | Product size |
| | ADIPOQ/rs2241766 | F: GCAGCTCCTAGCCGTAGACTCTGCTG
R: GGAGGTCTGTGATGAAAGAGGCC
FG: TGCTATTAGCTCTGCCCGAG
FT: TGCTATTAGCTCTGCCCGAT | 372 bp
226 bp |
| | ADIPOQ/rs1501299 | F: CCTGGTGAGAAGGGTGAGAA
R: AGATGCAGCAAAGCCAAAGT
FG: TGATATAAACTATATGAATG
FT: TGATATAAACTATATGAATT | 241 bp
168 bp |
| | TCF7L2/rs7903146 | F: GCCTCAAAACCTAGCACAGC
R: GTGAAGTGCCCAAGCTTCTC
FC: GCTAAGCACTTTTTAGATGC
FT: GCTAAGCACTTTTTAGATGT | 220 bp
176 bp |
| | TCF7L2/rs12255372 | F: CTTGAGGTGTACTGGAAACTAAGGC
R: CTGTCTATTTGGCATTCAAATGGA
FG: GGAATATCCAGGCAAGAACG
FT: GGAATATCCAGGCAAGAACT | 251 bp
130 bp |
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SNP: single nucleotide polymorphism; F: forward; R: reverse.
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