Phytotoxic Activity of Ocimum tenuiflorum Extracts on Germination and Seedling Growth of Different Plant Species

Table 1

The equations used to calculate different germination indices.

Germination parameters	Equations	References
Germination percent (GP)	GP=NumberofgerminatedseedsatfinalcountTotalnumberofseedssetsforbioassay×100	Global method
Germination index (GI)	𝐺GI=^𝑇𝑇𝑡; Or; NumberofgerminatedseedsDaysoffirstcount+⋯+NumberofgerminatedseedsDaysoflastorfinalcount	AOSA [13]
Time required for 50% germination (𝑇50)	𝑇50=𝑡𝑖+(𝑁/2)−𝑛𝑖𝑡𝑖−𝑡𝑗𝑛𝑖−𝑛𝑗 ,; where 𝑁 is the final number of germination and 𝑛𝑖,𝑛𝑗 cumulative numbers of seeds germinated by adjacent counts at times 𝑡𝑖 and 𝑡𝑗 when 𝑛𝑖<𝑁/2<𝑛𝑗	Coolbear et al. [14] modified by Farooq et al. [15]
Mean germination time (MGT)	MGT=∑𝑇𝑖/𝑁𝑖∑𝑁𝑖 ; here, 𝑁𝑖 = number of newly germinated seeds at time 𝑇𝑖	Ellis and Roberts [16]
Seedling vigour index (SVI)	()SVI=Seedlinglengthmm×Germinationpercent100	Islam et al. [17]
Speed of emergence (SE)	SE=NumberofgerminatedseedsatthestartingdayofgermiantionNumberofgerminatedseedsatthefinaldaysofmeasurement×100	Modified from Islam et al. [17]
Germination energy (GE)	GE=PercentageofgerminatedseedsatthestartingdayofgermiantionTotalnumberofseedssetsforbioassay×100	Modified from Ruan et al. [18]
Coefficient of the rate of germination (CRG)	𝑁CRG=^1+𝑁2+𝑁3+⋯+𝑁𝑛𝑁1×𝑇1+𝑁2×𝑇2+𝑁3×𝑇3+⋯+𝑁𝑛×𝑇𝑛×100,	Bewley and Black [19], Chiapusio et al. [20]
	where 𝑁1 = number of germinated seeds on time 𝑇1 ,; 𝑁2 = number of germinated seeds on time 𝑇2 ,; 𝑁𝑛 = number of germinated seeds on time 𝑇𝑛