Highly Effective Ex Vivo Gene Manipulation to Study Kidney Development Using Self-Complementary Adenoassociated Viruses
Transduction of embryonic kidney in vitro with different serotypes of scAAV. After incubation of the E12.5 kidney rudiments with different serotypes of scAAV at 4°C for 6 hours, the kidneys were cultured at 37°C for 24 hours. (a) EGFP (green) shows the gene expression of scAAV. The basement membrane marker laminin (red) shows both the UB and nephrons. Bar = 200 μm. (b) The relative EGFP fluorescence intensity was measured with ImageJ. All serotypes were tested for three times, with 3 kidney rudiments at each time.