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The Scientific World Journal
Volume 2014, Article ID 706189, 5 pages
http://dx.doi.org/10.1155/2014/706189
Research Article

Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions

1Endodontics Postgraduate Program, Faculty of Dentistry, The Autonomous University of San Luis Potosí, Manuel Nava 2, 78290 San Luis Potosí, SLP, Mexico
2Basic Sciences Laboratory, Faculty of Dentistry, The Autonomous University of San Luis Potosí, Manuel Nava 2, 78290 San Luis Potosí, SLP, Mexico
3Faculty of Engineering, The Autonomous University of San Luis Potosí, Manuel Nava 8, 78290 San Luis Potosí, SLP, Mexico
4Department of Endodontics and Department of Pediatric Dentistry, School of Dentistry, University of Washington, 6222 NE 74th Street, Seattle, 98115 WA, USA

Received 14 July 2014; Accepted 28 August 2014; Published 13 October 2014

Academic Editor: Louis M. Lin

Copyright © 2014 Ana Maria Gonzalez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. To evaluate a structurally mature E. faecalis biofilm developed under anaerobic/dynamic conditions in an in vitro system. Methods. An experimental device was developed using a continuous drip flow system designed to develop biofilm under anaerobic conditions. The inoculum was replaced every 24 hours with a fresh growth medium for up to 10 days to feed the system. Gram staining was done every 24 hours to control the microorganism purity. Biofilms developed under the system were evaluated under the scanning electron microscope (SEM). Results. SEM micrographs demonstrated mushroom-shaped structures, corresponding to a mature E. faecalis biofilm. In the mature biofilm bacterial cells are totally encased in a polymeric extracellular matrix. Conclusions. The proposed in vitro system model provides an additional useful tool to study the biofilm concept in endodontic microbiology, allowing for a better understanding of persistent root canal infections.