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The Scientific World Journal
Volume 2014, Article ID 726567, 10 pages
http://dx.doi.org/10.1155/2014/726567
Research Article

Transcripts of Anthocyanidin Reductase and Leucoanthocyanidin Reductase and Measurement of Catechin and Epicatechin in Tartary Buckwheat

1Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea
2Department of Bioscience and Biotechnology, University of Suwon, San 2-2 Wauri Bongdameup, Hwasung 445-743, Republic of Korea
3Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
4Department of Bio-Environmental Chemistry, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea
5Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan
6Department of Business Administration, Sahmyook University, Hwarangro 815, Nowon-gu, Seoul 139-742, Republic of Korea

Received 24 August 2013; Accepted 13 November 2013; Published 27 January 2014

Academic Editors: C. Lu, M. Tadege, and Y. Wu

Copyright © 2014 Yeon Bok Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) play an important role in the monomeric units biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin in several plants. The aim of this study was to clone ANR and LAR genes involved in PAs biosynthesis and examine the expression of these two genes in different organs under different growth conditions in two tartary buckwheat cultivars, Hokkai T8 and T10. Gene expression was carried out by quantitative real-time RT-PCR, and catechin and epicatechin content was analyzed by high performance liquid chromatography. The expression pattern of ANR and LAR did not match the accumulation pattern of PAs in different organs of two cultivars. Epicatechin content was the highest in the flowers of both cultivars and it was affected by light in only Hokkai T8 sprouts. ANR and LAR levels in tartary buckwheat might be regulated by different mechanisms for catechin and epicatechin biosynthesis under light and dark conditions.