Leucaena leucocephala Fruit Aqueous Extract Stimulates Adipogenesis, Lipolysis, and Glucose Uptake in Primary Rat Adipocytes

Table 1

List of probes used in Real-Time PCR.

Gene name	Gene symbol/gene aliases	Assay ID	Accession number
Protein kinase B	AKT1/AKT	Rn00583646_m1	NM_033230.1
Sterol regulatory element binding factor 1	Srebf1/Srebp1	Rn01495772_g1	AF286470.2
Hormone sensitive lipase	Lipe/HSL	Rn00689222_m1	NM_012859.1
Phosphatidylinositol-3-kinase	Pik3r1/PI3KA	Rn00564547_m1	NM_013005.1
Solute carrier family 2 (facilitated glucose transporter), member 4	Slc2a4/GLUT4	Rn00562597_m1	NM_012751.1

General abbreviation of the genes and their respective assay ID and accession numbers were obtained from the Applied Biosystems website. The assay ID indicates “Applied Biosystems gene expression assays” inventoried kits with patented primer and TaqMan probe mix. Assay ID with “Rn” prefix is defined as “Rattus norvegicus.” All gene expression assay kits listed here are FAM/MGB probed. Eukaryotic 18S rRNA was used as an endogenous control.