A Proteomics Analysis to Evaluate Cytotoxicity in NRK-52E Cells Caused by Unmodified Nano-Fe₃O₄

Table 2

Statistic classification of related proteins in NRK-52E cell lines with Fe₃O₄ NPs treatment.

UNIPROT accession^a	UNIPROT accession; number^a	Protein identification	Molecular mass (kDa)^b	Number of peptides^b	Ratio^c
Cell death and apoptosis related proteins
APAF_RAT	Q9EPV5	Apoptotic protease-activating factor 1	146.1	2	780.9
PDC10_RAT	Q6NX65	Programmed cell death protein 10	25.0	2	233.2
LEG1_RAT	P11762	Galectin-1	15.5	5	1.75
PDC6I_RAT	Q9QZA2	Programmed cell death 6-interacting protein	99.2	n.d.	n.d.
Ras-related proteins
RAB7L_RAT	Q63481	Ras-related protein Rab-7L1	23.7	2	3.2
RAB1A_RAT	Q6NYB7	Ras-related protein Rab-1A	23.4	13	2.3
RAB2A_RAT	P05712	Ras-related protein Rab-2A	24.1	3	1.6
RAC1_RAT	Q6RUV5	Ras-related C3 botulinum toxin substrate 1	22.4	2	1.2
RAP1A_RAT	P62836	Ras-related protein Rap-1A	21.8	n.d.	n.d.
RAB7A_RAT	P09527	Ras-related protein Rab-7a	24.3	n.d.	n.d.
Glutathione related proteins
GSHR_RAT	P70619	Glutathione reductase (Fragment)	47.8	2	3.1
GSTM1_RAT	P04905	Glutathione S-transferase Mu 1	26.7	2	2.8
GSTM2_RAT	P08010	Glutathione S-transferase Mu 2	26.5	3	2.4
GSTP1_RAT	P04906	Glutathione S-transferase P	24.1	6	1.5
GSTA3_RAT	P04904	Glutathione S-transferase alpha-3	25.9	4	1.2
Chaperone proteins
HSBP1_RAT	Q8K3X8	Heat shock factor-binding protein 1	8.7	2	2.8
HS90B_RAT	P34058	Heat shock protein HSP 90-beta	86.0	32	2.7
SERPH_RAT	P29457	Serpin H1	47.7	30	2.5
HS90A_RAT	P82995	Heat shock protein HSP 90-alpha	87.7	31	2.4
HSP74_RAT	O88600	Heat shock 70 kDa protein 4	97.0	2	2.2
HSP7C_RAT	P63018	Heat shock cognate 71 kDa protein	72.8	40	1.9
PDIA4_RAT	P38659	Protein disulfide-isomerase A4	75.1	9	1.8
CH60_RAT	P63039	60 kDa heat shock protein, mitochondrial	62.6	29	1.6
CH10_RAT	P26772	10 kDa heat shock protein, mitochondrial	11.2	3	1.6
GRP78_RAT	P06461	78 kDa glucose-regulated protein	74.4	19	1.6
ENPL_RAT	Q66HD0	Endoplasmin	95.4	17	1.6
PDIA1_RAT	P04785	Protein disulfide-isomerase	59.1	24	1.6
ERP29_RAT	P52555	Endoplasmic reticulum resident protein 29	29.3	3	1.6
PDIA6_RAT	Q63081	Protein disulfide-isomerase A6	49.6	4	1.5
PDIA3_RAT	P11598	Protein disulfide-isomerase A3	58.6	17	1.3

^aProtein accession and protein accession number were received from the UNIPROT database available online: http://www.uniprot.org/uniprot (accessed on May 25, 2014).
^bMolecular weight and number of protein peptides according to the Swiss-Prot database in the Mascot search engine.
^cRatio values of each protein according to protein list in the Mascot Distiller.