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The Scientific World Journal
Volume 2014, Article ID 763108, 6 pages
http://dx.doi.org/10.1155/2014/763108
Research Article

Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity

1The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin’an 311300, China
2School of Agricultural and Food Science, Zhejiang A&F University, Lin’an 311300, China
3Qingyuan Forestry Bureau of Zhejiang, Qingyuan 323800, China

Received 10 November 2013; Accepted 24 December 2013; Published 12 February 2014

Academic Editors: C. Cravo-Laureau and K. Ohmiya

Copyright © 2014 Li Fen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In order to screen lignocellulose-degrading superior mushroom strains ten strains of mushrooms (Lentinus edodes939, Pholiota nameko, Lentinus edodes868, Coprinus comatus, Macrolepiota procera, Auricularia auricula, Hericium erinaceus, Grifola frondosa, Pleurotus nebrodensis, and Shiraia bambusicola) were inoculated onto carboxymethylcellulose agar-Congo red plates to evaluate their ability to produce carbomethyl cellulase (CMCase). The results showed that the ratio of transparent circle to mycelium circle of Hericium erinaceus was 8.16 higher than other strains. The filter paper culture screening test showed that Hericium erinaceus and Macrolepiota procera grew well and showed extreme decomposition of the filter paper. When cultivated in guaiacol culture medium to detect their abilities to secrete laccase, Hericium erinaceus showed the highest ability with the largest reddish brown circles of 4.330 cm. CMCase activity determination indicated that Coprinus comatus and Hericium erinaceus had the ability to produce CMCase with 33.92 U/L on the 9th day and 22.58 U/L on the 10th day, respectively, while Coprinus comatus and Pleurotus nebrodensis had the ability to produce laccase with 496.67 U/L and 489.17 U/L on the 16th day and 18th day. Based on the results, Coprinus comatus might be the most promising lignocellulose-degrading strain to produce both CMCase and laccase at high levels.