TY - JOUR A2 - Sivaprasad, S. A2 - Zhong, Y. AU - Kara, Selcuk AU - Gencer, Baran AU - Karaca, Turan AU - Tufan, Hasan Ali AU - Arikan, Sedat AU - Ersan, Ismail AU - Karaboga, Ihsan AU - Hanci, Volkan PY - 2014 DA - 2014/01/30 TI - Protective Effect of Hesperetin and Naringenin against Apoptosis in Ischemia/Reperfusion-Induced Retinal Injury in Rats SP - 797824 VL - 2014 AB - Purpose. Hesperetin and naringenin are naturally common flavonoids reported to have antioxidative effects. This study was performed to investigate whether either hesperetin or naringenin has a protective effect against apoptosis on retinal ischemia/reperfusion (I/R) injury. Methods. Retinal I/R was induced by increasing the intraocular pressure to 150 mmHg for 60 minutes. Thirty-three male Wistar albino rats were randomised into 5 groups named control, I/R + sham, I/R + solvent (DMSO), I/R + hesperetin, and I/R + naringenin. Animals were given either hesperetin, naringenin, or the solvent intraperitoneally immediately following reperfusion. Thickness of retinal layers and retinal cell apoptosis were detected by histological analysis, tunel assay, and immunohistochemistry assay. Results. Hesperetin and naringenin attenuated the I/R-induced apoptosis of retinal cells in the inner and outer nuclear cells of the rat retina. Retinal layer thickness of the naringenin treatment group was significantly thicker than that of the hesperetin, sham, and solvent groups (P<0.05). Conclusions. Hesperetin and naringenin can prevent harmful effects induced by I/R injury in the rat retina by inhibiting apoptosis of retinal cells, which suggests that those flavanones have a therapeutic potential for the protection of ocular ischemic diseases. SN - 2356-6140 UR - https://doi.org/10.1155/2014/797824 DO - 10.1155/2014/797824 JF - The Scientific World Journal PB - Hindawi Publishing Corporation KW - ER -