Research Article

Clinicopathological and Molecular Findings in a Case of Canine Anaplasma phagocytophilum Infection in Northern Italy

Figure 1

(a) ML tree based on the GroEL alignment. The following reference A. phagocytophilum strains detected in several hosts from various parts of the world were obtained from GenBank and included in the molecular analysis: America lineage, accession numbers: AY848750; AY848752; AY848749; AF172163; AY848751; AY219849; DQ680012; Europe 1 lineage, accession numbers: AF033101; GQ452227; EU381151; EU381150; EU381152; EU982549; AY281849; AF482760; U96735; AY529490; AF478563; AF548386; HM057224; Europe 2 lineage, accession numbers: EU552921; EU552919; EU552912; AY220468; EU552915; EU552923; and Europe 3 lineage, accession numbers: EU552922; AY281818; AF383227; AF478561. To assess support for individual nodes, bootstrap resampling values were estimated with 1000 replicates. (b) Blood smear (May-Grunwald Giemsa staining) showing A. phagocytophilum morulae (black arrows) in the cytoplasm of neutrophil granulocytes of Case 1.
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