Molten Globule-Like Partially Folded State of Bacillus licheniformis α-Amylase at Low pH Induced by 1,1,1,3,3,3-Hexafluoroisopropanol

Table 1

Physical characteristics of different conformational states of BLA.

Variable	Native state	Acid-denatured state	HFIP-induced state
MRE222nm (deg·cm^2·dmol^−1)	−7557	−4649	−8615
% Helix^a	~17	~8	~21
Fluorescence intensity	871.2	241.8	259.8
Emission maximum (𝜆ex=280 nm)	337 nm	344 nm	341 nm
ANS fluorescence intensity	33	200	481
Emission maximum (𝜆ex=380 nm)	448 nm	474 nm	477 nm
Cooperativity (thermal transition)	Yes	No	No

aCalculated by the method of Chen et al. [32].