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The Scientific World Journal
Volume 2014, Article ID 825370, 6 pages
http://dx.doi.org/10.1155/2014/825370
Research Article

Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts

1Novel Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, Iran
2Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, Iran
3Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz 51656-65811, Iran
4Medical Philosophy and History Research Center, Tabriz University of Medical Sciences, Tabriz 371-51665, Iran

Received 7 August 2013; Accepted 24 October 2013; Published 14 January 2014

Academic Editors: Z. Liu and H. Verhoeven

Copyright © 2014 Mahdi Mojarrab et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro  β-hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC50 values of 1.36 ± 0.01 and 1.83 ± 0.03 mg/mL and IC90 values of 2.12 ± 0.04 and 2.62 ± 0.09 mg/mL for A. armeniaca and A. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of A. armeniaca and four fractions from DCM extract of A. aucheri showed potent antimalarial activity with reducing IC50 and IC90 values compared to extracts. The most potent fraction belonged to DCM extract of A. armeniaca with IC50 and IC90 values of 0.47 ± 0.006 and 0.71 ± 0.006 mg/mL, respectively.