878162.fig.002a
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878162.fig.002b
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878162.fig.002c
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878162.fig.002d
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878162.fig.002e
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878162.fig.002f
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878162.fig.002g
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878162.fig.002h
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Figure 2: Immunohistochemical analysis of BrdU retention in matrix and outer root sheath region. BrdU staining in hair follicles from 7-week-old C57BL/6 mice treated with water, 100°C and 4°C AAE at 100x magnification. ((a)–(c)) Vertical section BrdU incorporation cell in matrix part in the water, 100°C AAE and 4°C AAE group (open arrows indicated BrdU-positive cells). ((d)–(f)) Transverse sections BrdU incorporation cell in outer root sheath part in the water, 100°C AAE and 4°C AAE groups (diamond arrows indicated BrdU-positive cells). (g) Number of BrdU-positive cell in a fixed area with a width of 300 μm. (h) Schematic diagram of a hair follicle showing key components. Abbreviations: HS: hair shaft; M: hair matrix; IRS: inner root sheath; ORS: outer root sheath. Scale bars are 100 μm.