Figure 3: Immunohistochemical staining of BrdU incorporation in S-phase in anagen. ((a)–(c)) Paraffin sections from a 7-week-old C57BL/6 mouse injected with bromodeoxyuridine 48 h before necropsy, at magnification 100x. LRC could be seen in the matrix and the follicle papilla after labeling during the onset of anagen. Note the high proliferation rate (number of brown nuclei) in AAE-treated animals ((b) and (c)) compared to the control (a), with (d) showing the number of S-phase cells during anagen phase in different hair follicle regions. M: matrix, FP: follicle papilla, and HS: hair shaft. Scale bars are 100 μm.